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Impacts of Polyacrylamide Concentration on Protein Experiments

Polyacrylamide is a common reagent in the molecular biology lab as it is used in the formation of gels used to study proteins in techniques such as Western blotting and polyacrylamide gel electrophoresis (PAGE). Many scientists take advantage offalse

5 Tips for Reducing Non-specific Signal on Western Blots

Anyone who does western blots routinely knows the feeling: you test a new antibody and are filled with anticipation as your film lurches out of the X-OMAT. Seconds later, your hopes are crushed as you realize your samples have been lost to an uglyfalse

How current, voltage, and power settings affect SDS-PAGE

If you are new to Western blotting — or trying a new protocol for the first time — you’ll need to optimize the electrophoresis conditions.

Learn Western Blotting with These Open-Access Articles

Western blotting is a nearly ubiquitous procedure among biological research labs worldwide. From its initial introduction in the late 1970s to today, it has made and continues to make countless contributions to the advancement of science andfalse

5 Questions to Ask Before Purchasing an Antibody

Your literature review is almost done, your cell or animal model is established, and your research question is solidified. Now all that’s left is designing your experiment — and ordering your supplies.

Selecting the proper antibody for yourfalse

Why You (Maybe) Shouldn’t Fractionate That Western Blot Sample

Sample prep is common (and often overlooked) source of error when it comes to troubleshooting Western blots. One step in particular — fractionation — is suggested to reduce the “cellular debris,” but may in reality be harming your sample.

No Bands? 7 Quick Western Blot Fixes

 

We've all been there: You're at the end of your experiment and your Western blot protocol — the one that worked 24 hours earlier — is suddenly giving you fits. Try as you might, you just can't replicate your results and get that publicationfalse

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