Polyacrylamide is a common reagent in the molecular biology lab as it is used in the formation of gels used to study proteins in techniques such as Western blotting and polyacrylamide gel electrophoresis (PAGE). Many scientists take advantage offalse
Anyone who does western blots routinely knows the feeling: you test a new antibody and are filled with anticipation as your film lurches out of the X-OMAT. Seconds later, your hopes are crushed as you realize your samples have been lost to an uglyfalse
If you are new to Western blotting — or trying a new protocol for the first time — you’ll need to optimize the electrophoresis conditions.
Western blotting is a nearly ubiquitous procedure among biological research labs worldwide. From its initial introduction in the late 1970s to today, it has made and continues to make countless contributions to the advancement of science andfalse
Your literature review is almost done, your cell or animal model is established, and your research question is solidified. Now all that’s left is designing your experiment — and ordering your supplies.
Selecting the proper antibody for yourfalse
Sample prep is common (and often overlooked) source of error when it comes to troubleshooting Western blots. One step in particular — fractionation — is suggested to reduce the “cellular debris,” but may in reality be harming your sample.