Sample prep is common (and often overlooked) source of error when it comes to troubleshooting Western blots. One step in particular — fractionation — is suggested to reduce the “cellular debris,” but may in reality be harming your sample.
We've all been there: You're at the end of your experiment and your Western blot protocol — the one that worked 24 hours earlier — is suddenly giving you fits. Try as you might, you just can't replicate your results and get that publicationfalse
More than 90 percent of researchers still opt for traditional, paperbound notebooks to record their research (1). But the number looking to switch to electronic systems is quickly increasing. Electronic lab notebooks (ELNs) provide benefits in theirfalse