The Bioss Blog

News, updates, and analysis from the world of research. Interested in having your work with Bioss' products featured on the site? Email marketing@biossusa.com for more information.

Be sure to follow us on Twitter and Facebook for regular updates.

Tips for Reducing Non-specific Staining in IHC

Immunohistochemistry (IHC) can be a powerful tool for visualizing antigens of interest within the context of cells and tissues. But what can you do when non-specific staining in your IHC is obscuring your results and incurring your PI’s disdain?false

Impacts of Polyacrylamide Concentration on Protein Experiments

Polyacrylamide is a common reagent in the molecular biology lab as it is used in the formation of gels used to study proteins in techniques such as Western blotting and polyacrylamide gel electrophoresis (PAGE). Many scientists take advantage offalse

5 Tips for Reducing Non-specific Signal on Western Blots

Anyone who does western blots routinely knows the feeling: you test a new antibody and are filled with anticipation as your film lurches out of the X-OMAT. Seconds later, your hopes are crushed as you realize your samples have been lost to an uglyfalse

Our Comprehensive Guide to Performing Double Immunofluorescence

In attempting to characterize cellular and molecular processes, it often becomes necessary to label — either through immunofluorescence or immunohistochemistry — two or more targets in the same sample. This type of labeling provides insight intofalse

Fluorescent vs. Chromogenic Detection in Immunohistochemistry

Antibody detection for IHC typically occurs through either a fluorescent or chromogenic reaction. While the goal of each technique is similar — to label targets with high spatial resolution and low background — both methods have benefits andfalse

The Best Science Gifts for Kids this Season

'Tis the season of giving (and for using words like 'tis).

Instead of doling out for another pair of bad Christmas socks (okay, you can send those too), how about giving something more meaningful those nieces and nephews you haven’t seen sincefalse

Why You (Maybe) Shouldn’t Fractionate That Western Blot Sample

Sample prep is common (and often overlooked) source of error when it comes to troubleshooting Western blots. One step in particular — fractionation — is suggested to reduce the “cellular debris,” but may in reality be harming your sample.

1 2