Antibody detection for IHC typically occurs through either a fluorescent or chromogenic reaction. While the goal of each technique is similar — to label targets with high spatial resolution and low background — both methods have benefits andfalse
'Tis the season of giving (and for using words like 'tis).
Instead of doling out for another pair of bad Christmas socks (okay, you can send those too), how about giving something more meaningful those nieces and nephews you haven’t seen sincefalse
Sample prep is common (and often overlooked) source of error when it comes to troubleshooting Western blots. One step in particular — fractionation — is suggested to reduce the “cellular debris,” but may in reality be harming your sample.
We've all been there: You're at the end of your experiment and your Western blot protocol — the one that worked 24 hours earlier — is suddenly giving you fits. Try as you might, you just can't replicate your results and get that publicationfalse