IgGy's Blog

Our Comprehensive Guide to Performing Double Immunofluorescence

In attempting to characterize cellular and molecular processes, it often becomes necessary to label — either through immunofluorescence or immunohistochemistry — two or more targets in the same sample. This type of labeling provides insight intofalse

Read More

Fluorescent vs. Chromogenic Detection in Immunohistochemistry

Antibody detection for IHC typically occurs through either a fluorescent or chromogenic reaction. While the goal of each technique is similar — to label targets with high spatial resolution and low background — both methods have benefits andfalse

Read More

The Best Science Gifts for Kids this Season

'Tis the season of giving (and for using words like 'tis).

Instead of doling out for another pair of bad Christmas socks (okay, you can send those too), how about giving something more meaningful those nieces and nephews you haven’t seen sincefalse

Read More

Why You (Maybe) Shouldn’t Fractionate That Western Blot Sample

Sample prep is common (and often overlooked) source of error when it comes to troubleshooting Western blots. One step in particular — fractionation — is suggested to reduce the “cellular debris,” but may in reality be harming your sample.

Read More

No Bands? 7 Quick Western Blot Fixes

 

We've all been there: You're at the end of your experiment and your Western blot protocol — the one that worked 24 hours earlier — is suddenly giving you fits. Try as you might, you just can't replicate your results and get that publicationfalse

Read More
1 2