Immunohistochemistry (IHC) can be a powerful tool for visualizing antigens of interest within the context of cells and tissues. But what can you do when non-specific staining in your IHC is obscuring your results and incurring your PI’s disdain?false
Polyacrylamide is a common reagent in the molecular biology lab as it is used in the formation of gels used to study proteins in techniques such as Western blotting and polyacrylamide gel electrophoresis (PAGE). Many scientists take advantage offalse
Anyone who does western blots routinely knows the feeling: you test a new antibody and are filled with anticipation as your film lurches out of the X-OMAT. Seconds later, your hopes are crushed as you realize your samples have been lost to an uglyfalse
In attempting to characterize cellular and molecular processes, it often becomes necessary to label — either through immunofluorescence or immunohistochemistry — two or more targets in the same sample. This type of labeling provides insight into thefalse
Antibody detection for IHC typically occurs through either a fluorescent or chromogenic reaction. While the goal of each technique is similar — to label targets with high spatial resolution and low background — both methods have benefits andfalse
'Tis the season of giving (and for using words like 'tis).
Instead of doling out for another pair of bad Christmas socks (okay, you can send those too), how about giving something more meaningful those nieces and nephews you haven’t seen sincefalse
Sample prep is common (and often overlooked) source of error when it comes to troubleshooting Western blots. One step in particular — fractionation — is suggested to reduce the “cellular debris,” but may in reality be harming your sample.