Both of monoclonal and polyclonal antibodies are extensively used in IHC/IF assays. However, the staining results for a given monoclonal antibodies are quite unpredictable in different samples as compared to the polyclonal antibodies. It is veryfalse
Immunohistochemistry (IHC) can be a powerful tool for visualizing antigens of interest within the context of cells and tissues. But what can you do when non-specific staining in your IHC is obscuring your results and incurring your PI’s disdain?false