IgGy's Blog

Our Comprehensive Guide to Performing Double Immunofluorescence

In attempting to characterize cellular and molecular processes, it often becomes necessary to label — either through immunofluorescence or immunohistochemistry — two or more targets in the same sample. This type of labeling provides insight intofalse

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Fluorescent vs. Chromogenic Detection in Immunohistochemistry

Antibody detection for IHC typically occurs through either a fluorescent or chromogenic reaction. While the goal of each technique is similar — to label targets with high spatial resolution and low background — both methods have benefits andfalse

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How current, voltage, and power settings affect SDS-PAGE

If you are new to Western blotting — or trying a new protocol for the first time — you’ll need to optimize the electrophoresis conditions.

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