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How to rapidly extract proteins from recalcitrant plant tissues

 

WHICH IS BETTER, BSA vs. NON-FAT MILK, in WESTERN BLOT?

 

Frozen Sample Preparation for Immunohistochemical Assays

 

Isotype Controls in Flow Cytometry

 

Why do many researchers prefer polyclonal antibodies for an IHC/IF assay?

Both of monoclonal and polyclonal antibodies are extensively used in IHC/IF assays. However, the staining results for a given monoclonal antibodies are quite unpredictable in different samples as compared to the polyclonal antibodies. It is veryfalse

Tips for Reducing Non-specific Staining in IHC

Immunohistochemistry (IHC) can be a powerful tool for visualizing antigens of interest within the context of cells and tissues. But what can you do when non-specific staining in your IHC is obscuring your results and incurring your PI’s disdain?false

Impacts of Polyacrylamide Concentration on Protein Experiments

Polyacrylamide is a common reagent in the molecular biology lab as it is used in the formation of gels used to study proteins in techniques such as Western blotting and polyacrylamide gel electrophoresis (PAGE). Many scientists take advantage offalse

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