Both of monoclonal and polyclonal antibodies are extensively used in IHC/IF assays. However, the staining results for a given monoclonal antibodies are quite unpredictable in different samples as compared to the polyclonal antibodies. It is veryfalse
Immunohistochemistry (IHC) can be a powerful tool for visualizing antigens of interest within the context of cells and tissues. But what can you do when non-specific staining in your IHC is obscuring your results and incurring your PI’s disdain?false
Polyacrylamide is a common reagent in the molecular biology lab as it is used in the formation of gels used to study proteins in techniques such as Western blotting and polyacrylamide gel electrophoresis (PAGE). Many scientists take advantage offalse
In attempting to characterize cellular and molecular processes, it often becomes necessary to label — either through immunofluorescence or immunohistochemistry — two or more targets in the same sample. This type of labeling provides insight intofalse
Antibody detection for IHC typically occurs through either a fluorescent or chromogenic reaction. While the goal of each technique is similar — to label targets with high spatial resolution and low background — both methods have benefits andfalse
If you are new to Western blotting — or trying a new protocol for the first time — you’ll need to optimize the electrophoresis conditions.