In attempting to characterize cellular and molecular processes, it often becomes necessary to label — either through immunofluorescence or immunohistochemistry — two or more targets in the same sample. This type of labeling provides insight intofalse
Antibody detection for IHC typically occurs through either a fluorescent or chromogenic reaction. While the goal of each technique is similar — to label targets with high spatial resolution and low background — both methods have benefits andfalse
If you are new to Western blotting — or trying a new protocol for the first time — you’ll need to optimize the electrophoresis conditions.