The Bioss Blog

News, updates, and analysis from the world of research. Interested in having your work with Bioss' products featured on the site? Email marketing@biossusa.com for more information.

Be sure to follow us on Twitter and Facebook for regular updates.

Tips for Reducing Non-specific Staining in IHC

Immunohistochemistry (IHC) can be a powerful tool for visualizing antigens of interest within the context of cells and tissues. But what can you do when non-specific staining in your IHC is obscuring your results and incurring your PI’s disdain?false

Impacts of Polyacrylamide Concentration on Protein Experiments

Polyacrylamide is a common reagent in the molecular biology lab as it is used in the formation of gels used to study proteins in techniques such as Western blotting and polyacrylamide gel electrophoresis (PAGE). Many scientists take advantage offalse

Our Comprehensive Guide to Performing Double Immunofluorescence

In attempting to characterize cellular and molecular processes, it often becomes necessary to label — either through immunofluorescence or immunohistochemistry — two or more targets in the same sample. This type of labeling provides insight intofalse

Fluorescent vs. Chromogenic Detection in Immunohistochemistry

Antibody detection for IHC typically occurs through either a fluorescent or chromogenic reaction. While the goal of each technique is similar — to label targets with high spatial resolution and low background — both methods have benefits andfalse

How current, voltage, and power settings affect SDS-PAGE

If you are new to Western blotting — or trying a new protocol for the first time — you’ll need to optimize the electrophoresis conditions.

1