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Tips for Reducing Non-specific Staining in IHC

Immunohistochemistry (IHC) can be a powerful tool for visualizing antigens of interest within the context of cells and tissues. But what can you do when non-specific staining in your IHC is obscuring your results and incurring your PI’s disdain?false

Impacts of Polyacrylamide Concentration on Protein Experiments

Polyacrylamide is a common reagent in the molecular biology lab as it is used in the formation of gels used to study proteins in techniques such as Western blotting and polyacrylamide gel electrophoresis (PAGE). Many scientists take advantage offalse

5 Tips for Reducing Non-specific Signal on Western Blots

Anyone who does western blots routinely knows the feeling: you test a new antibody and are filled with anticipation as your film lurches out of the X-OMAT. Seconds later, your hopes are crushed as you realize your samples have been lost to an uglyfalse

Fluorescent vs. Chromogenic Detection in Immunohistochemistry

Antibody detection for IHC typically occurs through either a fluorescent or chromogenic reaction. While the goal of each technique is similar — to label targets with high spatial resolution and low background — both methods have benefits andfalse

5 Powerful Tools for Lab Management

More than 90 percent of researchers still opt for traditional, paperbound notebooks to record their research (1). But the number looking to switch to electronic systems is quickly increasing. Electronic lab notebooks (ELNs) provide benefits infalse

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