Immunohistochemistry (IHC) can be a powerful tool for visualizing antigens of interest within the context of cells and tissues. But what can you do when non-specific staining in your IHC is obscuring your results and incurring your PI’s disdain?false
Systemic lupus erythematosus is a chronic autoimmune disorder that can affect nearly any organ system in the body. A common symptom is lupus nephritis, which occurs when the disease attacks the kidneys causing inflammation, one of the leading causesfalse
Hepatocellular carcinoma (HCC) — the most common primary liver cancer in adults — currently has poor prognosis in most patients. Previous research has shown that, by activating or silencing specific genes, researchers can gain insights into thefalse
In attempting to characterize cellular and molecular processes, it often becomes necessary to label — either through immunofluorescence or immunohistochemistry — two or more targets in the same sample. This type of labeling provides insight into thefalse
Antibody detection for IHC typically occurs through either a fluorescent or chromogenic reaction. While the goal of each technique is similar — to label targets with high spatial resolution and low background — both methods have benefits andfalse
Your literature review is almost done, your cell or animal model is established, and your research question is solidified. Now all that’s left is designing your experiment — and ordering your supplies.
Selecting the proper antibody for your researchfalse