No Bands? 7 Quick Western Blot Fixes

No Bands? 7 Quick Western Blot Fixes

 

We've all been there: You're at the end of your experiment and your Western blot protocol — the one that worked 24 hours earlier — is suddenly giving you fits. Try as you might, you just can't replicate your results and get that publication quality image. It's time to start troubleshooting. But where do you start?

Here are some possible issues (and fixes) for when you don't see any bands on your blot:

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5 Powerful Tools for Lab Management

5 Powerful Tools for Lab Management

More than 90 percent of researchers still opt for traditional, paperbound notebooks to record their research (1). But the number looking to switch to electronic systems is quickly increasing. Electronic lab notebooks (ELNs) provide benefits in their security, reproducibility, and collaborative potential. Additionally, such systems are better suited to manage large inventories and datasets than traditional notebooks. While many PIs (especially in academia) consider the cost of such systems a major hurdle in switching, there are actually a number of free and low-cost options available. Here are some of the most popular products on the market today:

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Patricia Goldman-Rakic: Pioneer of the Prefrontal Cortex

Patricia Goldman-Rakic: Pioneer of the Prefrontal Cortex
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Christian de Duve: Father of Autophagy

Christian de Duve: Father of Autophagy

Belgian cytologist and biochemist Christian de Duve discovered lysosomes (the digestive organelles of the cell) and peroxisomes (organelles that are the site of metabolic processes involving hydrogen peroxide). In 1974, de Duve shared the Nobel Prize in Physiology or Medicine with Albert Claude and George E. Palade “for their discoveries concerning the structural and functional organization of the cell.” He coined the term “autophagy” during a conference along with endocytosis and exocytosis. De Duve was led to work on the topic because it combined two of his major interests, glucagon and lysosomes.

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Testing, testing, 1, 2, 3: New knockout validation coming for select antibodies

Testing, testing, 1, 2, 3: New knockout validation coming for select antibodies

As a researcher, you expect extraordinary products.

Bioss strives to meet your expectations every day, which is why we're proud to announce our new initiative to begin providing knockout (KO) validation on many of our 11,000+ products.

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